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Catalog No: OKCD09212
Size:96WELLS
Price: $800.00
SKU
OKCD09212
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Datasheets/ManualsPrintable datasheet for AMELX ELISA Kit (Human) (OKCD09212)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amelogenin, X-Linked (AMELX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amelogenin, X-Linked (AMELX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amelogenin, X-Linked (AMELX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amelogenin, X-Linked (AMELX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amelogenin, X-Linked (AMELX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amelogenin, X-Linked (AMELX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
AMELX Microplate96 Wells (12 x 8 Well strips)
AMELX Lyophilized Standard2
100X AMELX HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.057ng/mL
Gene SymbolAMELX
Gene Full Nameamelogenin X-linked
Alias SymbolsAI1E;AIH1;ALGN;amelogenin (amelogenesis imperfecta 1, X-linked);amelogenin (X chromosome, amelogenesis imperfecta 1);amelogenin, X isoform;AMG;AMGL;AMGX.
NCBI Gene Id265
Protein NameAmelogenin, X isoform
Description of TargetPlays a role in biomineralization. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.
Uniprot IDQ99217
Protein Accession #NP_001133.1
Nucleotide Accession #NM_001142.2
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