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Catalog No: OKEH04331
Size:96T
Price: $525.00
SKU
OKEH04331
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AMD1 ELISA Kit (Human) (OKEH04331) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AMD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AMD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AMD1 captured in the well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.1% (n = 20)
Mean Inter-assay CV%: <= 9.5% (n = 20)
ELISA Kit Component
ComponentAmount
AMD1 Microplate96 Wells (12 x 8 Well Strips)
AMD1 Standard2 x 2 ng
Sample Diluent1 x 20 mL
AMD1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.38 ng/mL
SpecificityNatural and recombinant Human S-adenosylmethionine decarboxylase proenzyme
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAMD1
Gene Full Nameadenosylmethionine decarboxylase 1
Alias SymbolsADOMETDC;AMD;S-adenosylmethionine decarboxylase 1;S-adenosylmethionine decarboxylase proenzyme;SAMDC.
NCBI Gene Id262
Protein NameP17707
Description of TargetS-adenosylmethionine decarboxylase proenzyme
Uniprot IDhttps://www.uniprot.org/uniprot/P17707
Protein Accession #NP_001625.2
Nucleotide Accession #NM_001634.5
Protein Size (# AA)334
Molecular Weight38 kDa
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