AMBP ELISA Kit (Human) (OKCD06145)

Catalog No: OKCD06145

Size:96WELLS

AMBP ELISA Kit (Human) (OKCD06145) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Microglobulin (a1M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Microglobulin (a1M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Microglobulin (a1M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-1-Microglobulin (a1M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 12.5-800pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Microglobulin (a1M) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Microglobulin (a1M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AMBP Microplate: 96 Wells (12 x 8 Well strips)
  • AMBP Lyophilized Standard: 2
  • 100X AMBP HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine and other biological fluids
• Sensitivity: < 6.1pg/mL

Target Info

• Gene Symbol: AMBP
• Gene Full Name: alpha-1-microglobulin/bikunin precursor
• Alias Symbols: A1M;bikunin;complex-forming glycoprotein heterogeneous in charge;EDC1;growth-inhibiting protein 19;HCP;HI30;IATIL;inter-alpha-trypsin inhibitor light chain;ITI;ITIL;ITILC;protein AMBP;protein HC;trypstatin;uristatin;uronic-acid-rich protein;UTI.
• NCBI Gene Id: 259
• Protein Name: Protein AMBP
• Description of Target: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
• Uniprot ID: P02760
• Protein Accession #: NP_001624.1
• Nucleotide Accession #: NM_001633.3