AMBP ELISA Kit (Human) (OKBB01120)

Catalog No: OKBB01120

Size:96 wells

AMBP ELISA Kit (Human) (OKBB01120) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human Bikunin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Bikunin has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: A206-N352) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Bikunin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Bikunin amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	38	243	418	36	264	406
  Standard deviation	2.35	16.03	25.08	2.48	18.48	25.17
  CV(%)	6.2	6.6	6	6.9	7	6.2

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human AMBP Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human AMBP Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human AMBP Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	38	40	35	40	38	2.04	5.3%
  Sample 2	243	252	272	236	250	13.51	5.4%
  Sample 3	418	447	460	414	434	19.35	4.4%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
  O.D.	0.107	0.165	0.201	0.296	0.475	0.736	1.236	1.790

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human Bikunin
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A206-N352
• Description: For Quantitative Detection of human Bikunin in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: AMBP
• Gene Full Name: alpha-1-microglobulin/bikunin precursor
• Alias Symbols: Protein AMBP; Alpha-1-microglobulin; Protein HC; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in charge; Inter-alpha-trypsin inhibitor light chain; ITI-LC; Bikunin; EDC1; HI-30; Uronic-acid-rich protein; Trypstatin; AMBP; HCP; ITIL
• NCBI Gene Id: 259
• Protein Name: Protein AMBP
• Description of Target: Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.
• Uniprot ID: P02760
• Protein Accession #: NP_001624.1
• Nucleotide Accession #: NM_001633.3