Size:96 Wells
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ALS2 ELISA Kit (Human) (OKWB05427)

Catalog#: OKWB05427
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration 2.5 Hours
Kit Principle Aviva Systems Biology ALS2 ELISA Kit (Human) (OKWB05427) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for ALS2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALS2 captured in the well.
Kit Range 0.313-20 ng/mL
Kit Reproducibility Intra-assay Precision: 3 samples with known low, middle and high level ALS2 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Serping1 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Component
Anti-ALS2 Microplate96 Wells (12 x 8 Well Strips)
ALS2 Lyophilized Standard2 x 20 ng
100X Biotinylated ALS2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma and other biological fluids
Sensitivity 0.188 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol ALS2
Official Gene Full Name alsin Rho guanine nucleotide exchange factor
Alias Symbols ALS2CR6, Alsin, ALSJ, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 6 protein, Amyotrophic lateral sclerosis 2 protein, FLJ31851, IAHSP, KIAA1563, MGC87187, PLSJ
NCBI Gene Id 57679
Protein Name Alsin
Description of Target May act as a GTPase regulator. Controls survival and growth of spinal motoneurons.
Swissprot Id Q96Q42
Protein Accession # NP_001129217
Nucleotide Accession # NM_020919
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ALS2.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ALS2.
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