ALPL ELISA Kit (Mouse) : 96 Wells (OKEH00758)

Catalog No: OKEH00758

Size:96T

Mouse ALPL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ALPL ELISA Kit (Mouse) (OKEH00758) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ALPL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ALPL and incubated. The ALPL found in the sample or standards competes with the biotinylated ALPL for limited binding sites on the immobilized anti-human ALPL antibody. Excess unbound biotinylated ALPL and sample or standard ALPL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ALPL captured in the well and inversely proportional to the amount of ALPL which was contained in the sample or standard.
• ELISA Kit Range: 3.12-200U/L
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.1% (n = 20)
  Mean Inter-assay CV%: < 10.4% (n = 20)
• ELISA Kit Component:
  • Anti-ALPL Microplate: 96 Wells (12 x 8 Well strips)
  • ALPL Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X ALPL-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 1.61U/L
• Specificity: Natural and recombinant Mouse Alkaline phosphatase, tissue-nonspecific isozyme
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Alpl
• Gene Full Name: alkaline phosphatase, liver/bone/kidney
• Alias Symbols: Ak;Akp;Akp2;Akp-2;Alkaline phosphatase 2;alkaline phosphatase 2, liver;Alkaline phosphatase liver/bone/kidney isozyme;alkaline phosphatase, tissue-nonspecific isozyme;ALP;APTNAP;T;TNAP;TNSALP.
• NCBI Gene Id: 11647
• Protein Name: P09242
• Description of Target: Alkaline phosphatase, tissue-nonspecific isozyme
• Uniprot ID: https://www.uniprot.org/uniprot/P09242
• Protein Accession #: NP_001274101.1
• Nucleotide Accession #: NM_001287172.1
• Protein Size (# AA): 524
• Molecular Weight: 57 kDa