Catalog No: OKEH00757
Size:96 Wells
Price: $525.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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ALPL ELISA Kit (Human) : 96 Wells (OKEH00757)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00757
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ALPL ELISA Kit (Human) (OKEH00757) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ALPL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ALPL and incubated. The ALPL found in the sample or standards competes with the biotinylated ALPL for limited binding sites on the immobilized anti-human ALPL antibody. Excess unbound biotinylated ALPL and sample or standard ALPL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ALPL captured in the well and inversely proportional to the amount of ALPL which was contained in the sample or standard.
ELISA Kit Range3.12 - 200U/L
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
Anti-ALPL Microplate96 Wells (12 x 8 Well strips)
ALPL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X ALPL-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
SpecificityNatural and recombinant Human Alkaline phosphatase, tissue-nonspecific isozyme

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Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolALPL
Gene Full Namealkaline phosphatase, liver/bone/kidney
NCBI Gene Id249
Protein NameAlkaline phosphatase, tissue-nonspecific isozyme
Description of TargetThis gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]
Uniprot IDP05186
Protein Accession #NP_000469.3
Nucleotide Accession #NM_000478.5
Protein Size (# AA)524
Molecular Weight57 kDa
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