Catalog No: OKEH00757
Size:96T
Price: $525.00
SKU
OKEH00757
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00757
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ALPL ELISA Kit (Human) (OKEH00757) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ALPL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ALPL and incubated. The ALPL found in the sample or standards competes with the biotinylated ALPL for limited binding sites on the immobilized anti-human ALPL antibody. Excess unbound biotinylated ALPL and sample or standard ALPL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ALPL captured in the well and inversely proportional to the amount of ALPL which was contained in the sample or standard.
ELISA Kit Range3.12-200U/L
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-ALPL Microplate96 Wells (12 x 8 Well strips)
ALPL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X ALPL-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity1.34U/L
SpecificityNatural and recombinant Human Alkaline phosphatase, tissue-nonspecific isozyme
Publications

Effects of the Highly COX-2-Selective Analgesic NSAID Etoricoxib on Human Periodontal Ligament Fibroblasts during Compressive Orthodontic Mechanical Strain. Mediators Inflamm. 2019, 2514956 (2019). 30983880

The role of mechanotransduction versus hypoxia during simulated orthodontic compressive strain-an in vitro study of human periodontal ligament fibroblasts. Int J Oral Sci. 11, 33 (2019). 31685804

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Gene SymbolALPL
Gene Full Namealkaline phosphatase, biomineralization associated
Alias Symbolsalkaline phosphatase liver/bone/kidney isozyme;alkaline phosphatase, liver/bone/kidney;alkaline phosphatase, tissue-nonspecific isozyme;APTNAP;AP-TNAP;HOPS;HPPA;HPPC;HPPI;HPPO;liver/bone/kidney-type alkaline phosphatase;tissue non-specific alkaline phosphatase;tissue-nonspecific ALP;TNALP;TNAP;TNSALP.
NCBI Gene Id249
Protein NameP05186
Description of TargetAlkaline phosphatase, tissue-nonspecific isozyme
Uniprot IDhttps://www.uniprot.org/uniprot/P05186
Protein Accession #NP_000469.3
Nucleotide Accession #NM_000478.5
Protein Size (# AA)524
Molecular Weight57 kDa
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