Catalog No: OKEH00757
Size:96T
Price: $525.00
SKU
OKEH00757
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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ALPL ELISA Kit (Human) : 96 Wells (OKEH00757)
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology ALPL ELISA Kit (Human) (OKEH00757) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ALPL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ALPL and incubated. The ALPL found in the sample or standards competes with the biotinylated ALPL for limited binding sites on the immobilized anti-human ALPL antibody. Excess unbound biotinylated ALPL and sample or standard ALPL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ALPL captured in the well and inversely proportional to the amount of ALPL which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 3.12-200U/L | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.5% (n = 20) Mean Inter-assay CV%: < 8.9% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 1.34U/L | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Alkaline phosphatase, tissue-nonspecific isozyme | ||||||||||||||||||||||
Publications | Effects of the Highly COX-2-Selective Analgesic NSAID Etoricoxib on Human Periodontal Ligament Fibroblasts during Compressive Orthodontic Mechanical Strain. Mediators Inflamm. 2019, 2514956 (2019). 30983880 The role of mechanotransduction versus hypoxia during simulated orthodontic compressive strain-an in vitro study of human periodontal ligament fibroblasts. Int J Oral Sci. 11, 33 (2019). 31685804 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA | ||||||||||||||||||||||
Description |
Gene Symbol | ALPL |
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Gene Full Name | alkaline phosphatase, biomineralization associated |
Alias Symbols | alkaline phosphatase liver/bone/kidney isozyme;alkaline phosphatase, liver/bone/kidney;alkaline phosphatase, tissue-nonspecific isozyme;APTNAP;AP-TNAP;HOPS;HPPA;HPPC;HPPI;HPPO;liver/bone/kidney-type alkaline phosphatase;tissue non-specific alkaline phosphatase;tissue-nonspecific ALP;TNALP;TNAP;TNSALP. |
NCBI Gene Id | 249 |
Protein Name | P05186 |
Description of Target | Alkaline phosphatase, tissue-nonspecific isozyme |
Uniprot ID | https://www.uniprot.org/uniprot/P05186 |
Protein Accession # | NP_000469.3 |
Nucleotide Accession # | NM_000478.5 |
Protein Size (# AA) | 524 |
Molecular Weight | 57 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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