ALPI Antibody (OAGA00947)

Catalog No: OAGA00947

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
OAGA00947 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SG xenograft, using alkaline phosphatase(intestinal)(OAGA00947) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
  Cellular Localization: Cell membrane; Lipid-anchor, GPI-anchor
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 3 and 206 (P09923) of Alkaline phosphatase (intestinal)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ALPI
• Gene Full Name: Intestinal-type alkaline phosphatase
• Alias Symbols: IAP
• NCBI Gene Id: 248
• Protein Name: Intestinal-type alkaline phosphatase
• Uniprot ID: P09923
• Protein Accession #: NP_001622.2
• Molecular Weight: 57 kDa