Catalog No: OASE00063-HRP
Size:200ug
Price: $379.00
SKU
OASE00063-HRP
Availability: Domestic: within 3-5 days delivery | International: 3-5 days
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Alpha A Crystallin Antibody : HRP (OASE00063-HRP)

Datasheets/ManualsPrintable datasheet for OASE00063-HRP
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Cow
Product FormatLiquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
ClonalityMonoclonal
Clone1H3.B8
IsotypeIgG1
HostMouse
ConjugationHRP: Horseradish Peroxidase
ApplicationWB, ELISA
Additional InformationBackground Info: Detects alpha-crystallin at ~20kDa. Does not cross-react with alphaB-crystallin, betaL-crystallin, betaH- crystallin, γ-crystallin, Hsp25, Hsp27 or Hsp47 proteins.
::Scientific Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).
::Certificate of Analysis: 0.5ug/ml was sufficient for detection of 100ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Reconstitution and StorageAll conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
ImmunogenNative Alpha Crystallin
PurificationProtein G Purified
Concentration0.6-0.7 mg/ml
Dilution1:2000 (WB)
Storage BufferPBS pH7.2, in 50% glycerol
Reference1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052.
2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453.
3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490
4. Horwitz J. (2003) Exp Eye Res. 76: 145-153.
5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924.
6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377.
7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215.
8. Yaung J., et al. (2007) Molecular Vision 13: 566-577.
Gene SymbolCRYAA
Gene Full Namecrystallin, alpha A
Alias SymbolsCRYA1, HSPB4, CTRCT9
NCBI Gene Id1409
Uniprot IDP02489
Protein Accession #NP_000385.1
  1. What is the species homology for "Alpha A Crystallin Antibody : HRP (OASE00063-HRP)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow".

  2. How long will it take to receive "Alpha A Crystallin Antibody : HRP (OASE00063-HRP)"?

    This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".

  3. What buffer format is "Alpha A Crystallin Antibody : HRP (OASE00063-HRP)" provided in?

    This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Alpha A Crystallin Antibody : HRP (OASE00063-HRP)"?

    This target may also be called "CRYA1, HSPB4, CTRCT9" in publications.

  5. What is the shipping cost for "Alpha A Crystallin Antibody : HRP (OASE00063-HRP)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Alpha A Crystallin Antibody : HRP (OASE00063-HRP)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Alpha A Crystallin Antibody : HRP (OASE00063-HRP)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Alpha A Crystallin Antibody : HRP (OASE00063-HRP)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "CRYAA"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CRYAA"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CRYAA"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CRYAA"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CRYAA"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CRYAA"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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