Alpha 2-Macroglobulin ELISA Kit (Human) (OKIA00052)

Catalog No: OKIA00052 (Formerly GWB-DM3742)

Size:96W

This product has been discontinued. We suggest the following suitable replacements: OKGD00005

Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.

Product Info

• Predicted Species Reactivity: Human
• Product Format: In Solution
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 80 Minutes
• ELISA Kit Principle: Aviva Systems Biology Alpha 2-Macroglobulin ELISA Kit (Human) (OKIA00052) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Alpha-2-macroglobulin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Alpha-2-macroglobulin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpha-2-macroglobulin captured in the well.
• ELISA Kit Range: 20 ng/ml - 640 ng/ml
• ELISA Kit Component:
  • Anti-Human Alpha 2-Macroglobulin Microplate: 96 Wells (12 x 8 Well Strips)
  • 20X Diluent Concentrate: 50 mL
  • 20X Wash Solution: 50 mL
  • 100X Enzyme-Antibody Conjugate: 150 uL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
  • Human Alpha 2-Macroglobulin Calibrator: 1
• Additional Information: Intended Use: The Alpha 2-Macroglobulin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 2-Macroglobulin in the serum and plasma of humans.
  Reagents:
  1. DILUENT CONCENTRATE (Running Buffer)
  One bottle containing 50 ml of a 5X concentrated diluent running buffer.
  2. WASH SOLUTION CONCENTRATE
  One bottle containing 50 ml of a 20X concentrated wash solution.
  3. ENZYME-ANTIBODY CONJUGATE 100X
  One vial containing 150 ul of affinity purified anti-Human Alpha 2-Macroglobulin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  4. CHROMOGEN-SUBSTRATE SOLUTION
  One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  5. STOP SOLUTION
  One vial containing 12 ml 0.3 M sulfuric acid.
  WARNING: Avoid contact with skin.
  6. ANTI-HUMAN A2M ELISA MICRO PLATE
  Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human A2M.
  7. HUMAN A2M CALIBRATOR
  One vial containing a lyophilized Human Alpha 2-Macroglobulin calibrator.
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 2.409 ng/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: A2M
• Gene Full Name: alpha-2-macroglobulin
• Alias Symbols: A2MD, CPAMD5, FWP007, S863-7
• NCBI Gene Id: 2
• Protein Name: Alpha-2-macroglobulin
• Description of Target: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
• Uniprot ID: P01023