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Catalog No: OKIA00052 (Formerly GWB-DM3742)
Size:1x96WELLPLATE
Price: $438.00
SKU
OKIA00052
Availability: Domestic: within 1 week delivery | International: 1 week
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Alpha 2-Macroglobulin ELISA Kit (Human) (OKIA00052)

This product has been discontinued. We suggest the following suitable replacements: OKGD00005
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration80 Minutes
ELISA Kit PrincipleAviva Systems Biology Alpha 2-Macroglobulin ELISA Kit (Human) (OKIA00052) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Alpha-2-macroglobulin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Alpha-2-macroglobulin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpha-2-macroglobulin captured in the well.
ELISA Kit Range20 ng/ml - 640 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Human Alpha 2-Macroglobulin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Alpha 2-Macroglobulin Calibrator1
Additional InformationIntended Use: The Alpha 2-Macroglobulin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 2-Macroglobulin in the serum and plasma of humans.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Alpha 2-Macroglobulin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN A2M ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human A2M.
7. HUMAN A2M CALIBRATOR
One vial containing a lyophilized Human Alpha 2-Macroglobulin calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity2.409 ng/ml
Predicted Homology Based on Immunogen SequenceHuman
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolA2M
Gene Full Namealpha-2-macroglobulin
Alias SymbolsA2MD, CPAMD5, FWP007, S863-7
NCBI Gene Id2
Protein NameAlpha-2-macroglobulin
Description of TargetIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Uniprot IDP01023
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