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Catalog No: OKIA00051 (Formerly GWB-D2CE63)
Size:1x96WELLPLATE
Price: $419.00
SKU
OKIA00051
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHuman
Product FormatIn Solution
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration130 Minutes
ELISA Kit PrincipleAviva Systems Biology Alpha 1-Microglobulin ELISA Kit (Human) (OKIA00051) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Protein AMBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Protein AMBP - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Protein AMBP captured in the well.
ELISA Kit Range12.5 ng/ml - 400 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Human alpha 1-Microglobulin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human alpha 1-Microglobulin Calibrator1
Additional InformationIntended Use: The Alpha 1 Microglobulin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 1 Microglobulin in biological fluid of Humans.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Alpha 1 Microglobulin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN A1M ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human A1M.
7. HUMAN A1M CALIBRATOR
One vial containing a lyophilized Human Alpha 1 Microglobulin calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity2.481 ng/ml
Predicted Homology Based on Immunogen SequenceHuman
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAMBP
Gene Full Namealpha-1-microglobulin/bikunin precursor
Alias SymbolsA1M, HCP, ITI, UTI, EDC1, HI30, ITIL, IATIL, ITILC
NCBI Gene Id259
Protein NameProtein AMBP
Description of TargetInter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
Uniprot IDP02760
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