Catalog No: OKIA00048 (Formerly GWB-5428A0)
Size:1x96WELLPLATE
Price: $400.00
SKU
OKIA00048
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKIA00048
Product Info
Predicted Species ReactivityHuman
Product FormatIn Solution
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration100 Minutes
ELISA Kit PrincipleAviva Systems Biology Alpha 1-Antitrypsin ELISA Kit (Human) (OKIA00048) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Alpha-1-antitrypsin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Alpha-1-antitrypsin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpha-1-antitrypsin captured in the well.
ELISA Kit Range7.81 ng/ml - 500 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Human Alpha 1-Antitrypsin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Alpha 1-Antitrypsin Calibrator1
Additional InformationIntended Use: The Alpha 1-Antitrypsin (A1AT) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring A1AT in biological fluid of humans.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Alpha 1-Antitrypsin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN ALPHA 1-ANTITRYPSIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human A1AT.
7. HUMAN ALPHA 1-ANTITRYPSIN CALIBRATOR
One vial containing a lyophilized Human Alpha 1-Antitrypsin calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.094 ng/ml
Predicted Homology Based on Immunogen SequenceHuman
Publications

Case study on human a1-antitrypsin: Recombinant protein titers obtained by commercial ELISA kits are inaccurate. Biotechnol J. 11, 1648-1656 (2016). 27731958

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSERPINA1
Gene Full Nameserpin family A member 1
Alias SymbolsPI, A1A, AAT, PI1, A1AT, nNIF, PRO2275, alpha1AT
NCBI Gene Id5265
Protein NameAlpha-1-antitrypsin
Description of TargetInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Uniprot IDP01009
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