ALOX15B ELISA Kit (Rat) (OKEH06184)

Catalog No: OKEH06184

Size:96T

Rat ALOX15B ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Alox15b ELISA Kit (Rat) (OKEH06184) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Alox15b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Alox15b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alox15b captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 100%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <4.7%, n = 20
  Mean Inter-Assay Precision - <7.5%, n = 20
• ELISA Kit Component:
  • ALOX15B Microplate: 96 Wells (12 x 8 Well strips)
  • ALOX15B Lyophilized Standard: 2
  • 100X Biotinylated ALOX15B Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.194 ng/mL
• Specificity: Natural and recombinant Rat Arachidonate 15-lipoxygenase B
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Alox15b
• Gene Full Name: arachidonate 15-lipoxygenase, type B
• Alias Symbols: 15-lipoxygenase 2;15-LOX-2;15-LOX-B;arachidonate 15-lipoxygenase B;arachidonate 15-lipoxygenase type II;arachidonate 15-lipoxygenase, second type;linoleate 13-lipoxygenase 15-LOb;polyunsaturated fatty acid lipoxygenase ALOX15B.
• NCBI Gene Id: 266604
• Protein Name: Q8K4F2
• Description of Target: Arachidonate 15-lipoxygenase B
• Uniprot ID: https://www.uniprot.org/uniprot/Q8K4F2
• Protein Accession #: NP_695213.1
• Nucleotide Accession #: NM_153301.2
• Protein Size (# AA): 677