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Catalog No: OKEH04836
Size:96T
Price: $525.00
SKU
OKEH04836
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology ALOX15B ELISA Kit (Human) (OKEH04836) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALOX15B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALOX15B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALOX15B captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
ALOX15B Microplate96 Wells (12 x 8 Well Strips)
ALOX15B Standard2
Sample Diluent1 x 20 mL
ALOX15B Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.098 ng/mL
SpecificityNatural and recombinant Human Arachidonate 15-lipoxygenase B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolALOX15B
Gene Full Namearachidonate 15-lipoxygenase type B
Alias Symbols15-lipoxygenase 2;15-LOX-2;15-LOX-B;15S-lipoxygenase;arachidonate 15-lipoxygenase 2;arachidonate 15-lipoxygenase type II;arachidonate 15-lipoxygenase, second type;arachidonate omega(6) lipoxygenase;linoleate 13-lipoxygenase 15-LOb;polyunsaturated fatty acid lipoxygenase ALOX15B.
NCBI Gene Id247
Protein NameO15296
Description of TargetArachidonate 15-lipoxygenase B
Uniprot IDhttps://www.uniprot.org/uniprot/O15296
Protein Accession #NP_001034219.1
Nucleotide Accession #NM_001039130.1
Protein Size (# AA)676
Molecular Weight76 kDa
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