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Catalog No: OKCD07352
Size:96WELLS
Price: $750.00
SKU
OKCD07352
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Datasheets/ManualsPrintable datasheet for ALOX15B ELISA Kit (Human) (OKCD07352)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 15-Lipoxygenase-2 (15-LO-2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 15-Lipoxygenase-2 (15-LO-2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 15-Lipoxygenase-2 (15-LO-2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 15-Lipoxygenase-2 (15-LO-2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 15-Lipoxygenase-2 (15-LO-2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 15-Lipoxygenase-2 (15-LO-2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
ALOX15B Microplate96 Wells (12 x 8 Well strips)
ALOX15B Lyophilized Standard2
100X ALOX15B HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.059ng/mL
Gene SymbolALOX15B
Gene Full Namearachidonate 15-lipoxygenase type B
Alias Symbols15-lipoxygenase 2;15-LOX-2;15-LOX-B;15S-lipoxygenase;arachidonate 15-lipoxygenase 2;arachidonate 15-lipoxygenase type II;arachidonate 15-lipoxygenase, second type;arachidonate omega(6) lipoxygenase;linoleate 13-lipoxygenase 15-LOb;polyunsaturated fatty acid lipoxygenase ALOX15B.
NCBI Gene Id247
Protein NameArachidonate 15-lipoxygenase B
Description of TargetNon-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells.
Uniprot IDO15296
Protein Accession #NP_001034219.1
Nucleotide Accession #NM_001039130.1
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