Catalog No: OKCD07349
Size:96WELLS
Price: $750.00
SKU
OKCD07349
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for ALOX15 ELISA Kit (Human) (OKCD07349)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Lipoxygenase (5-LO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Lipoxygenase (5-LO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Lipoxygenase (5-LO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 5-Lipoxygenase (5-LO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Lipoxygenase (5-LO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Lipoxygenase (5-LO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
ALOX15 Microplate96 Wells (12 x 8 Well strips)
ALOX15 Lyophilized Standard2
100X ALOX15 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.059ng/mL
Gene SymbolALOX5
Gene Full Namearachidonate 5-lipoxygenase
Alias Symbols5-LO;5-LOX;5LPG;arachidonic acid 5-lipoxygenase;leukotriene A4 synthase;LOG5;LOX-5;polyunsaturated fatty acid 5-lipoxygenase.
NCBI Gene Id240
Protein NameArachidonate 5-lipoxygenase
Description of TargetCatalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Uniprot IDP09917
Protein Accession #NP_001131.3
Nucleotide Accession #NM_001140.3
Write Your Own Review
You're reviewing:ALOX15 ELISA Kit (Human) (OKCD07349)
Your Rating
We found other products you might like!