Catalog No: OKEH08453
Size:96T
Price: $800.00
SKU
OKEH08453
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology ALOX15 ELISA Kit (Bovine) (OKEH08453) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALOX15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALOX15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALOX15 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.8% (n = 20)
Mean Inter-assay CV%: <=12.2% (n = 20)
ELISA Kit Component
ComponentAmount
ALOX15 Microplate96 Wells (12 x 8 Well strips)
ALOX15 Lyophilized Standard2
100X Biotinylated ALOX15 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Oxygenase and 14,15-leukotriene A4 synthase activity.
::Subcellular Location: Cytoplasm
Reconstitution and StorageStore as indicated in product manual.
Sensitivity21pg/mL
SpecificityNatural and recombinant Bovine Arachidonate 12-lipoxygenase, 12S-type
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolALOX15
Gene Full Namearachidonate 15-lipoxygenase
Alias Symbols12/15-lipoxygenase;12-LOX;12S-lipoxygenase;12S-LOX;15-LOX;ALOX12;arachidonate 12-lipoxygenase, 12S-type;arachidonate 12-lipoxygenase, leukocyte-type;arachidonate 15-lipoxygenase;arachidonate omega-6 lipoxygenase;erythroid cell-specific 15-lipoxygenase;hepoxilin A3 synthase Alox15;linoleate 13S-lipoxygenase;polyunsaturated fatty acid lipoxygenase ALOX15.
NCBI Gene Id282139
Protein NameP27479
Description of TargetArachidonate 15-lipoxygenase
Uniprot IDhttps://www.uniprot.org/uniprot/P27479
Protein Accession #NP_776926.1
Nucleotide Accession #NM_174501.2
Write Your Own Review
You're reviewing:ALOX15 ELISA Kit (Bovine) (OKEH08453)
Your Rating