ALOX12 Recombinant Protein (OPCD01256)

Catalog No: OPCD01256

ALOX12 Recombinant Protein (OPCD01256) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Human
• Product Format: Lyophilized PBS, pH7.4, containing 0.01% Sarkosyl, 1mM DTT, 5% Trehalose and Proclin300.
• Host: E.coli
• Conjugation: Unconjugated
• Application: SDS-PAGE, WB, ELISA, IP
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Phe352-Cys656
  Subcellular Location: Cytoplasm, cytosol. Membrane
• Reconstitution and Storage: Reconstitute in ddH₂O to the original concentration prior to lyophilization. If additional dilution is required, dilute further in the original base lyophilization buffer excluding stabilizers such as Sarkosyl, Proclin, Trehalose or Sucrose. Do not vortex. Store at 2-8C for one month. Aliquot and store at -80C for 12 months. Avoid repeated freeze/thaw cycles.
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 90%
• Source: Prokaryotic Expressed Recombinant
• Tag: N-terminal His Tag

Target Info

• Gene Symbol: ALOX12
• Gene Full Name: arachidonate 12-lipoxygenase, 12S type
• Alias Symbols: LOG12, 12-LOX, 12S-LOX
• NCBI Gene Id: 239
• Protein Name: Arachidonate 12-lipoxygenase, 12S-type
• Description of Target: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.
• Uniprot ID: P18054
• Protein Accession #: NP_000688.2
• Nucleotide Accession #: NM_000697.2
• Molecular Weight: 38 kDa