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Catalog No: OCOA17284
Size:20UG
Price: $169.00
SKU
OCOA17284
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ALOX12 Protein Lysate (OCOA17284)

Datasheets/ManualsPrintable datasheet for ALOX12 Protein Lysate (OCOA17284)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionALOX12 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolALOX12
Gene Full Namearachidonate 12-lipoxygenase, 12S type
Alias Symbols12-LOX;12S-lipoxygenase;12S-LOX;arachidonate (12S)-lipoxygenase;arachidonate (15S)-lipoxygenase;Arachidonate 12-lipoxygenase, 12S-type;arachidonate 15-lipoxygenase,15S-type;linoleate 13S-lipoxygenase;lipoxin synthase 12-LO;LOG12;platelet 12-LOX;platelet-type 12-lipoxygenase;platelet-type lipoxygenase 12;polyunsaturated fatty acid lipoxygenase ALOX12.
NCBI Gene Id239
Protein NameNM_000697, NP_000688, P18054
Description of TargetArachidonate 12-lipoxygenase, 12S-type, arachidonate 12-lipoxygenase, 12S-type [Homo sapiens], Homo sapiens arachidonate 12-lipoxygenase, 12S type (ALOX12), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P18054
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000688
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000697
Molecular Weight75.5 kDa
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