ALKBH1 Antibody (OACA06689)

Catalog No: OACA06689

Immunohistochemistry of paraffin-embedded human colon cancer at dilution 1:100

All lanes: Alkylated DNA repair protein alkB homolog 1 antibody at 9ug/ml+mouse kidney tissue
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 44kDa
Observed band size: 44kDa

All lanes: Alkylated DNA repair protein alkB homolog 1 antibody at 9ug/ml+mouse kidney tissue
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 44kDa
Observed band size: 44kDa

Immunohistochemistry of paraffin-embedded human colon cancer at dilution 1:100

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS with 0.02% sodium azide, 50% glycerol (pH 7.3)
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC, ELISA
• Reconstitution and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Recombinant human Alkylated DNA repair protein alkB homolog 1 protein (110-389AA)
• Purification: Antigen Affinity Purified
• Concentration: Varies by lot. See vial for exact concentration.
• Application Info: WB: 1:500~5000
  IHC: 1:20~200
• Reference: [1]N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB." Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012). [2]Human AlkB homologue 1 (ABH1) exhibits DNA lyase activity at abasic sites."Muller T.A., Meek K., Hausinger R.P.DNA Repair 9:58-65(2010). [3]Expression and sub-cellular localization of human ABH family molecules."Tsujikawa K., Koike K., Kitae K., Shinkawa A., Arima H., Suzuki T., Tsuchiya M., Makino Y., Furukawa T., Konishi N., Yamamoto H.J. Cell. Mol. Med. 11:1105-1116(2007).
• Publications:

  ALKBH1-demethylated DNA N6-methyladenine modification triggers vascular calcification via osteogenic reprogramming in chronic kidney disease. J Clin Invest. 131, NULL (2021)

Target Info

• Gene Symbol: ALKBH1
• Gene Full Name: alkB homolog 1, histone H2A dioxygenase
• Alias Symbols: ABH, ABH1, alkB, hABH, ALKBH
• NCBI Gene Id: 8846
• Protein Name: DNA demethylase ALKBH1
• Description of Target: Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation. Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
• Uniprot ID: Q13686
• Protein Accession #: NP_006011
• Nucleotide Accession #: NM_006020