ALKAL1 ELISA Kit (Human) (OKEH08334)

Catalog No: OKEH08334

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ALKAL1 ELISA Kit (Human) (OKEH08334) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALKAL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALKAL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALKAL1 captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.8% (n = 20)
  Mean Inter-assay CV%: <=11.2% (n = 20)
• ELISA Kit Component:
  • ALKAL1 Microplate: 96 Wells (12 x 8 Well strips)
  • ALKAL1 Lyophilized Standard: 2
  • 100X Biotinylated ALKAL1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Ligand for receptor tyrosine kinase LTK and perhaps receptor tyrosine kinase ALK; activation of ALK is reported conflictingly.
  Subcellular Location: Secreted
  Unigene: Hs.527111
  String: 9606.ENSP00000351345
  KEGG: hsa:389658
  Pfam: PF15129
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 15.8pg/mL
• Specificity: Natural and recombinant Human ALK and LTK ligand 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ALKAL1
• Gene Full Name: ALK and LTK ligand 1
• Alias Symbols: ALK and LTK ligand 1;AUGA;AUGB;AUG-beta;augmentor beta;augmentor-alpha;FAM150A;family with sequence similarity 150 member A;protein FAM150A;RPLK9433;UNQ9433.
• NCBI Gene Id: 389658
• Protein Name: Q6UXT8
• Description of Target: ALK and LTK ligand 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q6UXT8
• Protein Accession #: NP_997296.1
• Nucleotide Accession #: NM_207413.3