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Catalog No: OKCD04306
Size:96WELLS
Price: $625.00
SKU
OKCD04306
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Datasheets/ManualsPrintable datasheet for Alk-Smase ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Sphingomyelinase (Alk-Smase). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Sphingomyelinase (Alk-Smase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Sphingomyelinase (Alk-Smase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alkaline Sphingomyelinase (Alk-Smase) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Sphingomyelinase (Alk-Smase) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Sphingomyelinase (Alk-Smase) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Alk-smase Microplate96 Wells (12 x 8 Well strips)
Alk-smase Lyophilized Standard2
100X Alk-smase HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity0.051 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolALK-SMASE
Alias SymbolsAlk-SMase, Gm254, LOC238011
Protein NameEctonucleotide pyrophosphatase/phosphodiesterase 7
Uniprot IDA2A5N7
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