ALK-SMASE ELISA Kit (Human) (OKCD04449)

Catalog No: OKCD04449

Size:96WELLS

Human ALK-SMASE ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	84-94%	88-99%	82-102%	89-103%
  EDTA Plasma (n=5)	99-105%	86-95%	83-104%	78-101%
  Heparin Plasma (n=5)	87-96%	85-99%	79-98%	95-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Sphingomyelinase (Alk-Smase). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Sphingomyelinase (Alk-Smase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Sphingomyelinase (Alk-Smase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alkaline Sphingomyelinase (Alk-Smase) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	86-101	94
  EDTA Plasma (n=5)	80-89	86
  Heparin Plasma (n=5)	78-104	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Sphingomyelinase (Alk-Smase) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Sphingomyelinase (Alk-Smase) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Hpt Microplate: 96 Wells (12 x 8 Well strips)
  • Hpt Lyophilized Standard: 2
  • 100X Hpt HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: 1.39 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ENPP7
• Gene Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 7
• Alias Symbols: alkaline sphingomyelin phosphodiesterase;alkaline sphingomyelinase;ALK-SMase;ectonucleotide pyrophosphatase/phosphodiesterase family member 7;E-NPP 7;intestinal alkaline sphingomyelinase;NPP7;NPP-7.
• NCBI Gene Id: 339221
• Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 7
• Description of Target: Converts sphingomyelin to ceramide and phosphocholine (PubMed:12885774, PubMed:12671034, PubMed:15205117, PubMed:28292932). Has also phospholipase C activity and can cleave phosphocholine from palmitoyl lyso-phosphatidylcholine (PubMed:12885774). Does not have nucleotide pyrophosphatase activity (PubMed:12885774).
• Uniprot ID: Q6UWV6
• Protein Accession #: NP_848638.3
• Nucleotide Accession #: NM_178543.4