ALK Antibody (OAAF01512)

Catalog No: OAAF01512 (Formerly GWB-ASC886)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ALK Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human ALK.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: ALK antibody detects endogenous levels of total ALK protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:40000
• Reference: Morris S.W., Oncogene 14:2175-2188(1997).
  Morris S.W., Oncogene 15:2883-2883(1997).
  wahara T.,Oncogene 14:439-449(1997).

Target Info

• Gene Symbol: ALK
• Gene Full Name: ALK receptor tyrosine kinase
• Alias Symbols: ALK tyrosine kinase receptor;Anaplastic lymphoma kinase;anaplastic lymphoma receptor tyrosine kinase;CD246;CD246 antigen;mutant anaplastic lymphoma kinase;NBLST3.
• NCBI Gene Id: 238
• Protein Name: ALK tyrosine kinase receptor
• Description of Target: Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
• Uniprot ID: Q9UM73
• Protein Accession #: NP_004295.2
• Molecular Weight: 176 kDa