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Catalog No: OCOA09544
Size:20UG
Price: $179.00
SKU
OCOA09544
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ALG13 Protein Lysate (OCOA09544)

Datasheets/ManualsPrintable datasheet for ALG13 Protein Lysate (OCOA09544)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionALG13 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolALG13
Gene Full NameALG13 UDP-N-acetylglucosaminyltransferase subunit
Alias Symbolsasparagine-linked glycosylation 13 homolog;CDG1S;CXorf45;DEE36;EIEE36;GLT28D1;glycosyltransferase 28 domain-containing protein 1;hematopoietic stem/progenitor cells protein MDS031;MDS031;N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase;putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13;TDRD13;tudor domain containing 13;UDP-N-acetylglucosamine transferase subunit ALG13 homolog;YGL047W.
NCBI Gene Id79868
Protein NameNM_018466, NP_060936, Q9NP73, Q9NP73-2
Description of TargetHomo sapiens ALG13, UDP-N-acetylglucosaminyltransferase subunit (ALG13), transcript variant 2, mRNA, Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13, putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 isoform 2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/?query=Q9NP73+OR+Q9NP73-2
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_060936
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_018466
Molecular Weight18.2 kDa
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