Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH02567
Size:96T
Price: $425.00
SKU
OKEH02567
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02567
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Aldosterone ELISA Kit (OKEH02567) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Aldosterone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Aldosterone and incubated. The Aldosterone found in the sample or standards competes with the biotinylated Aldosterone for limited binding sites on the immobilized anti-Aldosterone antibody. Excess unbound biotinylated Aldosterone and sample or standard Aldosterone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Aldosterone captured in the well and inversely proportional to the amount of Aldosterone which was contained in the sample or standard.
ELISA Kit Range0.39-25ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Aldosterone Microplate96 Wells (12 x 8 Well strips)
Aldosterone Lyophilized Standard2 x 25 ng
Sample Diluent1 x 20 mL
100X Aldosterone-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: ALD
Target Name: Aldosterone
::Pubchem: 5839
::Chemical formula: C21H28O5
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.194 ng/mL
SpecificityNatural and recombinant General Aldosterone
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsALD, Aldosterone, ALD
Protein NameAldosterone
Description of TargetAldosterone is a yellow steroid hormone (mineralocorticoid family) produced by the outer-section (zona glomerulosa) of the adrenal cortex in the adrenal gland, and acts mainly on the distal tubules and collecting ducts of the nephron, the functioning unit of the kidney, to cause the conservation of sodium, secretion of potassium, increased water retention, and increased blood pressure. The overall effect of aldosterone is to increase reabsorption of ions and water in the kidney -- increasing blood volume and, therefore, increasing blood pressure. Steroid receptors are intracellular. The aldosterone mineralcorticoid receptor (MR) complex binds on the DNA to specific hormone response element, which leads to gene specific transcription.
Write Your Own Review
You're reviewing:Aldosterone ELISA Kit (OKEH02567)
Your Rating