ALDOA ELISA Kit (Human) (OKEH04675)

Catalog No: OKEH04675

Size:96T

Human ALDOA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology ALDOA ELISA Kit (Human) (OKEH04675) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALDOA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ALDOA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALDOA captured in well.
• ELISA Kit Range: 1.56-100U/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 7.0%
  Mean Inter-Assay CV: < 8.3%
• ELISA Kit Component:
  • Anti-ALDOA Microplate: 96 Wells (12 x 8 Well Strips)
  • ALDOA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • ALDOA Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: serum, Plasma , Urine ,tissue homogenates and Cell culture supernates and Other biological fluids
• Sensitivity: 0.78U/mL
• Specificity: Natural and recombinant Human Fructose-bisphosphate aldolase A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ALDOA
• Gene Full Name: aldolase, fructose-bisphosphate A
• Alias Symbols: ALDA;aldolase A, fructose-bisphosphate;epididymis secretory sperm binding protein Li 87p;fructose-1,6-bisphosphate triosephosphate-lyase;fructose-bisphosphate aldolase A;GSD12;HEL-S-87p;lung cancer antigen NY-LU-1;muscle-type aldolase.
• NCBI Gene Id: 226
• Protein Name: P04075
• Description of Target: Fructose-bisphosphate aldolase A
• Uniprot ID: https://www.uniprot.org/uniprot/P04075
• Protein Accession #: NP_000025.1
• Nucleotide Accession #: NM_000034.3
• Protein Size (# AA): 364
• Molecular Weight: 39 KDa