ALDH2 ELISA Kit (Human) (OKCD07919)

Catalog No: OKCD07919

Size:96WELLS

ALDH2 ELISA Kit (Human) (OKCD07919) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase, Mitochondrial (ALDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Dehydrogenase, Mitochondrial (ALDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Dehydrogenase, Mitochondrial (ALDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aldehyde Dehydrogenase, Mitochondrial (ALDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldehyde Dehydrogenase, Mitochondrial (ALDH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldehyde Dehydrogenase, Mitochondrial (ALDH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ALDH2 Microplate: 96 Wells (12 x 8 Well strips)
  • ALDH2 Lyophilized Standard: 2
  • 100X ALDH2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.124ng/mL

Target Info

• Gene Symbol: ALDH2
• Gene Full Name: aldehyde dehydrogenase 2 family member
• Alias Symbols: acetaldehyde dehydrogenase 2;aldehyde dehydrogenase 2 family (mitochondrial);aldehyde dehydrogenase, mitochondrial;ALDH class 2;ALDH-E2;ALDHI;ALDM;epididymis secretory sperm binding protein;liver mitochondrial ALDH;nucleus-encoded mitochondrial aldehyde dehydrogenase 2.
• NCBI Gene Id: 217
• Protein Name: Aldehyde dehydrogenase, mitochondrial
• Description of Target: Aldehyde dehydrogenase, mitochondrial
• Uniprot ID: P05091
• Protein Accession #: NP_000681.2
• Nucleotide Accession #: NM_000690.3