Catalog No: OKCD01161 (Formerly GWB-KBBYY4)
Size:96WELLS
Price: $675.00
SKU
OKCD01161
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Datasheets/ManualsPrintable datasheet for ALDH1A2 ELISA Kit (Human) (OKCD01161)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)90-98%98-105%88-102%96-105%
EDTA plasma(n=5)79-90%89-104%79-101%89-97%
heparin plasma(n=5)79-103%79-90%97-104%79-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-9988
EDTA plasma(n=5)87-10391
heparin plasma(n=5)91-9994
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ALDH1A2 Microplate96 Wells (12 x 8 Well Strips)
ALDH1A2 Lyophilized Standard2 x 40 ng
100X Biotinylated ALDH1A2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.215 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2). No significant cross-reactivity or interference between Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolALDH1A2
Gene Full Namealdehyde dehydrogenase 1 family member A2
Alias SymbolsAldehyde dehydrogenase family 1 member A2;RALDH 2;RALDH(II);RALDH2;RALDH2-T;retinal dehydrogenase 2;retinaldehyde-specific dehydrogenase type 2.
NCBI Gene Id8854
Protein NameRetinal dehydrogenase 2
Description of TargetRecognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently (By similarity).
Uniprot IDO94788
Protein Accession #NP_001193826
Nucleotide Accession #NM_001206897.1
Protein Size (# AA)518
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