ALDH1A1 ELISA Kit (Human) (OKCD08718)

Catalog No: OKCD08718

Size:96WELLS

ALDH1A1 ELISA Kit (Human) (OKCD08718) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ALDH1A1 Microplate: 96 Wells (12 x 8 Well strips)
  • ALDH1A1 Lyophilized Standard: 2
  • 100X ALDH1A1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.34ng/mL

Target Info

• Gene Symbol: ALDH3A1
• Gene Full Name: aldehyde dehydrogenase 3 family member A1
• Alias Symbols: Aldehyde dehydrogenase 3;Aldehyde dehydrogenase family 3 member A1;aldehyde dehydrogenase isozyme 3;aldehyde dehydrogenase type III;aldehyde dehydrogenase, dimeric NADP-preferring;ALDH3;ALDHIII;stomach aldehyde dehydrogenase.
• NCBI Gene Id: 218
• Protein Name: Aldehyde dehydrogenase, dimeric NADP-preferring
• Description of Target: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde (Probable). They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable). Oxidizes medium and long chain aldehydes into non-toxic fatty acids (PubMed:1737758). Preferentially oxidizes aromatic aldehyde substrates (PubMed:1737758). Comprises about 50 percent of corneal epithelial soluble proteins (By similarity). May play a role in preventing corneal damage caused by ultraviolet light (By similarity).
• Uniprot ID: P30838
• Protein Accession #: NP_000680.2
• Nucleotide Accession #: NM_000689.4