Catalog No: OKCD08716
Size:96WELLS
Price: $800.00
SKU
OKCD08716
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Shipping Info:
- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for ALDH1A1 ELISA Kit (Human) (OKCD08716) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.63ng/mL |
Gene Symbol | ALDH1A1 |
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Gene Full Name | aldehyde dehydrogenase 1 family member A1 |
Alias Symbols | acetaldehyde dehydrogenase 1;ALDC;aldehyde dehydrogenase 1, soluble;Aldehyde dehydrogenase family 1 member A1;Aldehyde dehydrogenase, cytosolic;aldehyde dehydrogenase, liver cytosolic;ALDH class 1;ALDH1;ALDH11;ALDH-E1;ALHDII;epididymis luminal protein 12;epididymis luminal protein 9;epididymis secretory sperm binding protein Li 53e;HEL12;HEL-9;HEL-S-53e;PUMB1;RALDH 1;RALDH1;retinal dehydrogenase 1;retinaldehyde dehydrogenase 1. |
NCBI Gene Id | 216 |
Protein Name | Retinal dehydrogenase 1 |
Description of Target | Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (PubMed:19296407, PubMed:26373694, PubMed:25450233). |
Uniprot ID | P00352 |
Protein Accession # | NP_000680.2 |
Nucleotide Accession # | NM_000689.4 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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