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Albumin ELISA Kit (Pig) (OKIA00185)

Catalog#: OKIA00185
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species Reactivity Pig
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Duration 100 Minutes
Kit Principle Aviva Systems Biology Albumin ELISA Kit (Pig) (OKIA00185) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serum albumin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serum albumin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serum albumin captured in the well.
Kit Range 12.5 ng/ml - 400 ng/ml
Kit Component
Anti-Pig Albumin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Pig Albumin Calibrator1
Additional Information Intended Use: The Albumin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Albumin in biological fluid of Pig.


One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 L of affinity purified anti- Pig Albumin antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.WARNING:
Avoid contact with skin.6. ANTI-PIG ALBUMIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Pig Albumin antibodies.7. PIG ALBUMIN CALIBRATOR
One vial containing a lyophilized Pig Albumin calibrator.
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma and other biological fluids
Sensitivity 2.258 ng/ml
Predicted Homology Based on Immunogen Sequence Pig
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Introduction Albumin (Alb) is an amazing polyfunctional protein contributing to homeostasis through mechanisms of hemodynamics, transport and nutrition. Albumin is found both intra and extravascularly in all mammals and many lower vertebrates. It is a molecule of about 67,000 daltons, synthesized by the liver. Normally only very trace amounts of albumin escape reabsorption by kidney glomeruli and is excreted into the urine. Many occult diseases can cause kidney damage which may result in excessive amounts of serum proteins, including albumin, to be excreted by the kidney and into the urine. This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids.
Alias Symbols Albumin, ALB
NCBI Gene Id 396960
Protein Name Serum albumin
Description of Target Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Swissprot Id P08835
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