Aviva Systems Biology office will be closed for Memorial Day - May 27, 2019.
Please go here for more info.

Search Antibody, Protein, and ELISA Kit Solutions

Albumin ELISA Kit (Human) (OKIA00046)

96 Wells
$339.00
In Stock
Request Bulk Order Quote

Application:
ELISA
Gene Symbol:
ALB
Official Gene Full Name:
albumin
NCBI Gene Id:
213
Alias Symbols:
Albumin, ALB
Description of Target:
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Protein Name:
Serum albumin
Swissprot Id:
P02768
Predicted Species Reactivity:
Human
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
1.900 ng/ml
Kit Range:
6.25 ng/ml - 200 ng/ml
Kit Duration:
100 Minutes
Kit Principle:
Aviva Systems Biology Albumin ELISA Kit (Human) (OKIA00046) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serum albumin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serum albumin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serum albumin captured in the well.
Kit Component:
ComponentAmount
Anti-Human Albumin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Albumin Calibrator1
Kit Detection Method:
Colorimetric, OD450 nm
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Product Format:
In Solution
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Additional Information:
Intended Use: The Albumin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Albumin in biological fluid of Human.
:::
Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Albuminumin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN ALBUMIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human Albumin.
7. HUMAN ALBUMIN CALIBRATOR
One vial containing a lyophilized Human Albumin calibrator.

Product Reviews

Protocol:
Tips Information:

Tell us what you think about this item!

Write A Review
    Please, wait...