Catalog No: OKCD01294
Size:96WELLS
Price: $750.00
SKU
OKCD01294
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Albumin Antibody ELISA Kit (Human) (OKCD01294)

Datasheets/ManualsPrintable datasheet for Albumin Antibody ELISA Kit (Human) (OKCD01294)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h, 30min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)89-103%94-102%83-103%80-91%
EDTA Plasma (n=5)85-97%95-105%89-102%93-105%
Heparin Plasma (n=5)87-101%91-101%81-103%90-102%
ELISA Kit PrincipleThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Albumin Antibody (Anti-ALB) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Albumin Antibody (Anti-ALB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-9387
EDTA Plasma (n=5)92-10197
Heparin Plasma (n=5)80-103101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Albumin Antibody (Anti-ALB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Albumin Antibody (Anti-ALB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Albumin antibody Microplate96 Wells (12 x 8 Well strips)
Albumin antibody Lyophilized Standard2 x XXX
100X Detector Antibody1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.27 ng/mL
Assay InfoAssay Method: Quantitative Reverse Capture Sandwich ELISA
Gene SymbolRHOA
Gene Full Nameras homolog family member A
Alias SymbolsAplysia ras-related homolog 12;ARH12;ARHA;EDFAOB;epididymis secretory sperm binding protein;oncogene RHO H12;Rho cDNA clone 12;RHO12;RHOH12;small GTP binding protein RhoA;transforming protein RhoA.
NCBI Gene Id387
Protein NameTransforming protein RhoA
Description of TargetRegulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436, PubMed:19403695).
Uniprot IDP61586
Nucleotide Accession #NM_000477.6
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