Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCA00226
Size:96 Wells
Price: $525.00
SKU
OKCA00226
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

ALB ELISA Kit (Rabbit) : 96 Wells (OKCA00226)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRabbit
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:25095%80-105%
Serum (n=4)1:50097%91-115%
Serum (n=4)1:100094%85-98%
Serum (n=4)1:200095%90-112%
ELISA Kit PrincipleAviva Systems Biology ALB ELISA Kit (Rabbit) (OKCA00226) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Rabbit ALB and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to ALB and incubated. ALB immobilized in the wells competes with the liquid phase ALB contained in the samples or standards for binding with the HRP-Conjugated ALB Antibody. Wells are washed removing unbound ALB, unbound HRP-Conjugated ALB Antibody or ALB/HRP-Conjugated ALB Antibody complex. HRP-Conjugated ALB Antibody bound to ALB immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated ALB Antibody captured in the well and inversely proportional to the amount of ALB which was contained in the sample or standard.
ELISA Kit Range0.273 ug/mL - 70 ug/mL
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean (ug/ml)20.43121.42419.77921.18719.29820.884
SD0.0130.0150.0110.0160.0270.018
CV%2.4552.8251.9932.9714.9213.37
ELISA Kit Component
ComponentAmount
Anti-ALB Microplate96 Well (12 x 8 Well Strips)
ALB Standard 1 x 200 uL
100X HRP-Conjugate1 x 60 uL
HRP-Conjugate Diluent1 x 20 mL
Sample Diluent2 x 20 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, urine
Sensitivity< 0.117 ug/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of rabbit albumin. No significant cross-reactivity or interference between rabbit albumin and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Gene SymbolALB
Gene Full Namealbumin
NCBI Gene Id100009195
Protein NameSerum albumin
Description of TargetAlbumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
Uniprot IDP49065
Protein Accession #NP_001075813
Nucleotide Accession #NM_001082344
Protein Size (# AA)608
Molecular Weight69 kDa
Write Your Own Review
You're reviewing:ALB ELISA Kit (Rabbit) : 96 Wells (OKCA00226)
Your Rating
We found other products you might like!