Size:96 Wells
$455.00
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SKU
OKCA00370
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ALB ELISA Kit (Pigeon) : 96 Wells (OKCA00370)

Catalog#: OKCA00370
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Pigeon
Kit Detection Method Colorimetric, OD450 nm
Kit Duration 2.5 Hours
Kit Principle Aviva Systems Biology ALB ELISA Kit (Pigeon) (OKCA00370) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for pigeon ALB has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for pigeon ALB is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample pigeon ALB captured in well.
Kit Range 2.19 ug/mL - 140 ug/mL
Kit Reproducibility Intra-assay: CV% < 8% (n = 20).Inter-assay: CV% < 10% (n = 20)
Kit Component
ComponentAmount
Anti-ALB Microplate96 Well (12 x 8 Well Strips)
ALB Standard (Lyophilized)2 x 140 ug
100X ALB Detection Antibody1 x 120 uL
100X HRP-Avidin1 x 120 uL
Detection Antibody Diluent1 x 15 mL
HRP-Avidin Diluent1 x 15 mL
Sample Diluent1 x 50 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type serum, plasma
Sensitivity < 0.55 ug/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of pigeon Alb. No significant cross-reactivity or interference between pigeon Alb and analogues was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between pigeon Alb and all the analogues, therefore, cross reaction may still exist.
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Gene Symbol ALB
Official Gene Full Name PREDICTED: Columba livia albumin
NCBI Gene Id 102091530
Protein Name PREDICTED: serum albumin
Protein Accession # XP_005498595
Nucleotide Accession # XM_005498538
Protein Size (# AA) 609
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ALB.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ALB.
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