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ALB ELISA Kit (Mouse) (OKEH03992)

96 Wells
$239.00
In Stock
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Predicted Species Reactivity:
Mouse
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology Alb ELISA Kit (Mouse) (OKEH03992) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Alb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Alb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alb captured in the well.
Kit Range:
78 - 5000pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: <5.8% (n = 20)
Mean Inter-assay CV%: <8.4% (n = 20)
Kit Component:
ComponentAmount
Anti-Alb Microplate96 Wells (12 x 8 Well Strips)
Alb Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Alb Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
29.6 pg/mL
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Mouse Serum albumin
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol:
ALB
Official Gene Full Name:
albumin
Alias Symbols:
Alb1, Alb-1, Serum albumin
NCBI Gene Id:
11657
Protein Name:
serum albumin
Description of Target:
This gene encodes albumin, an abundant plasma protein essential for maintaining oncotic pressure that functions as a carrier protein for various molecules such as steriods and fatty acids in blood. This gene is primarily expressed in liver where the encoded protein undergoes proteolytic processing before secretion into the plasma.
Swissprot Id:
P07724
Protein Accession #:
NP_033784.2
Nucleotide Accession #:
NM_009654.4
Protein Size (# AA):
608
Molecular Weight:
69 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ALB.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ALB.

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