ALB ELISA Kit (Horse) : 96 Wells (OKCA00357)

Catalog No: OKCA00357

Size:96 Wells

Horse ALB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Horse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:5,000	94%	80-105%
  Serum (n=4)	1:10,000	90%	82-106%
  Serum (n=4)	1:20,000	94%	89-115%
  Serum (n=4)	1:40,000	94%	88-106%

• ELISA Kit Principle: Aviva Systems Biology ALB ELISA Kit (Horse) (OKCA00357) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Horse ALB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ALB and incubated. The ALB found in the sample or standards competes with the biotinylated ALB for limited binding sites on the immobilized anti-Horse ALB antibody. Excess unbound biotinylated ALB and sample or standard ALB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ALB captured in the well and inversely proportional to the amount of ALB which was contained in the sample or standard.
• ELISA Kit Range: 1.25 ug/ml - 80 ug/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (μg/mL)	6.89	6.712	6.803	6.901	6.65	6.591
  SD	0.011	0.023	0.025	0.031	0.027	0.043
  CV%	1.657	3.423	3.731	4.775	4.069	6.401

• ELISA Kit Component:
  • Anti-ALB Microplate: 96 Well (12 x 8 Well Strips)
  • ALB Standard (Lyophilized): 2
  • 100X ALB-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 20 mL
  • Sample Diluent: 2 x 20 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma
• Sensitivity: 1.247 ug/ml
• Specificity: This assay has high sensitivity and excellent specificity for detection of horse
  albumin. No significant cross-reactivity or interference between horse albumin
  and analogues was observed
• Assay Info: Assay Methodology: Quantitative Competitive Immunoassay

Target Info

• Gene Symbol: ALB
• Gene Full Name: albumin
• NCBI Gene Id: 100034206
• Protein Name: Serum albumin
• Description of Target: Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
• Uniprot ID: P35747
• Protein Accession #: NP_001075972.1
• Nucleotide Accession #: NM_001082503.1
• Protein Size (# AA): 607
• Molecular Weight: 69 kDa