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Catalog No: OKCA00286
Size:96 Wells
Price: $525.00
SKU
OKCA00286
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ALB ELISA Kit (Guinea Pig) : 96 Wells (OKCA00286)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityGuinea Pig
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:1,00089%84-95%
Serum (n=4)1:2,00087%82-96%
Serum (n=4)1:4,00092%86-100%
Serum (n=4)1:8,00090%84-95%
ELISA Kit PrincipleAviva Systems Biology ALB ELISA Kit (Guinea) (OKCA00286) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Guinea ALB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ALB and incubated. The ALB found in the sample or standards competes with the biotinylated ALB for limited binding sites on the immobilized anti-Guinea ALB antibody. Excess unbound biotinylated ALB and sample or standard ALB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ALB captured in the well and inversely proportional to the amount of ALB which was contained in the sample or standard.
ELISA Kit Range0.156 ug/mL - 10 ug/mL
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean (μg/mL)1.1191.1081.1471.1671.1371.337
SD0.0310.0360.0350.040.0490.042
CV%4.6835.5155.4746.2537.5566.942
ELISA Kit Component
ComponentAmount
Anti-ALB Microplate96 Well (12 x 8 Well Strips)
ALB Standard 2 x 500 uL
100X ALB-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
Sample Diluent2 x 20 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity0.09 ug/ml
SpecificityThis assay has high sensitivity and excellent specificity for detection of guinea
pig albumin. No significant cross-reactivity or interference between guinea pig
albumin and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Gene SymbolALB
Gene Full Namealbumin
NCBI Gene Id100379232
Protein NamePreproalbumin
Description of TargetAlbumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
Uniprot IDQ6WDN9
Protein Accession #NP_001166576.1
Nucleotide Accession #NM_001173105.1
Protein Size (# AA)608
Molecular Weight69 kDa
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