ALB Chemi-Luminescent ELISA Kit (Human) (OKCD04526)

Catalog No: OKCD04526

Size:96WELLS

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Antibody detection|Chemiluminescence
• ELISA Kit Duration: 2h, 30min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Anti-Albumin Antibody (Anti-ALB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Anti-Albumin Antibody (Anti-ALB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Anti-Albumin Antibody (Anti-ALB) level in the sample or standard.
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Albumin Antibody (Anti-ALB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Albumin Antibody (Anti-ALB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ALB Microplate: 96 Wells (12 x 8 Well strips)
  • ALB Lyophilized Standard: 2
  • 100X ALB HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 9 mL
  • Substrate Diluent: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids

Target Info

• Gene Symbol: RHOA
• Gene Full Name: ras homolog family member A
• Alias Symbols: Aplysia ras-related homolog 12;ARH12;ARHA;EDFAOB;epididymis secretory sperm binding protein;oncogene RHO H12;Rho cDNA clone 12;RHO12;RHOH12;small GTP binding protein RhoA;transforming protein RhoA.
• NCBI Gene Id: 387
• Protein Name: P61586
• Description of Target: Transforming protein RhoA
• Uniprot ID: https://www.uniprot.org/uniprot/P61586
• Protein Accession #: NP_000468.1
• Nucleotide Accession #: NM_000477.6