alamarBlue Accessory Reagent (OOSA11224)

Catalog No: OOSA11224

Size:25ML

Product Info

• Product Format: Liquid
• Application: ELISA|FA|IF
  Test Principle :
  - Growing cells cause a chemical reduction of alamarBlue®.
  - Continued growth maintains a reduced environment.(fluorescent, red).
  - Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
  - Data may be collected using either fluorescence-based or absorbance-based instrumentation.
  - Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
  - Absorbance is monitored at 570nm and 600nm.
• Reconstitution and Storage: 2°C to 8°C

Target Info

• Description of Target: alamarBlue® is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi.
  alamarBlue® is an indicator dye, that incorporates an oxidation_reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue® assay is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi. Some variability in absorbance may occur between batches of AlamarBlue® but all batches should fall between 0.84 and 0.95 AU when measured between 600nm and 602nm on a spectrophotometer.