Alad ELISA Kit (Mouse) (OKEH04773)

Catalog No: OKEH04773

Size:96T

Mouse Alad ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Alad ELISA Kit (Mouse) (OKEH04773) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Alad has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Alad is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alad captured in well.
• ELISA Kit Range: 1.25-80ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Alad Microplate: 96 Wells (12 x 8 Well Strips)
  • Alad Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Alad Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.635 ng/mL
• Specificity: Natural and recombinant Mouse Delta-aminolevulinic acid dehydratase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Alad
• Gene Full Name: aminolevulinate, delta-, dehydratase
• Alias Symbols: ALADH;delta-aminolevulinic acid dehydratase;Lv;porphobilinogen synthase.
• NCBI Gene Id: 17025
• Protein Name: P10518
• Description of Target: Delta-aminolevulinic acid dehydratase
• Uniprot ID: https://www.uniprot.org/uniprot/P10518
• Protein Accession #: NP_001263375.1
• Nucleotide Accession #: NM_001276446.1
• Protein Size (# AA): 330
• Molecular Weight: 36 kDa