Catalog No: OKEH01480
Size:96T
Price: $525.00
SKU
OKEH01480
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ALAD ELISA Kit (Human) : 96 Wells (OKEH01480)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ALAD ELISA Kit (Human) (OKEH01480) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALAD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALAD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALAD captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 10.0% (n = 20)
ELISA Kit Component
ComponentAmount
ALAD Microplate96 Wells (12 x 8 Well strips)
ALAD Lyophilized Standard2
100X Biotinylated ALAD Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.78 ng/mL
SpecificityNatural and recombinant Human Delta-aminolevulinic acid dehydratase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolALAD
Gene Full Nameaminolevulinate dehydratase
Alias SymbolsALADH;aminolevulinate, delta-, dehydratase;delta-aminolevulinic acid dehydratase;PBGS;porphobilinogen synthase;testicular tissue protein Li 95.
NCBI Gene Id210
Protein NameP13716
Description of TargetDelta-aminolevulinic acid dehydratase
Uniprot IDhttps://www.uniprot.org/uniprot/P13716
Protein Accession #NP_000022.3
Nucleotide Accession #NM_000031.5
Protein Size (# AA)330
Molecular Weight36 kDa
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