Size:400ul
$310.00
Discontinued
SKU
OASE00377
Request Bulk
Order Quote
Aviva's
Satisfaction
Guarantee
Contact Us:
  • Toll Free: (888)880-0001
  • Phone: (858)552-6979
  • Email: info@avivasysbio.com
Shipping Info:
  • $40: Antibody & Protein in US
  • $50: 1-2 Kits in US
  • Contact us for international orders.

Conjugation Options

OASE00377-FITC Conjugated

OASE00377-HRP Conjugated

OASE00377-Biotin Conjugated

Akt2 (PKB beta) Antibody (OASE00377)

Catalog#: OASE00377
Inquire
This product is Genway GWB-0X0008
More Information
Predicted Species Reactivity Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus.
Clonality Polyclonal
Host Rabbit
Application WB
Additional Information Background Info: Detects a 65kDa protein, corresponding to the molecular mass of Akt2 PKBbeta on SDS PAGE immunoblots. Does not react with PKBalpha (Akt1) or PKBγ (Akt3).
:: Scientific Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the
phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
:: Certificate of Analysis: A 13000 dilution was sufficient for detection of PKBbeta in 20ug of HeLa cell lysate by ECL immunoblot analysis.
Reconstitution and Storage Store at -20C. Shipping in Blue Ice or 4C.
Replacement Item This antibody may replace item sc-116831 from Santa Cruz Biotechnology.
Immunogen A five residue synthetic peptide based on the human Akt2, coupled to KLH
Purification Rabbit immunoglobulin
Concentration 0.25mg/mL
Datasheets/Manuals Printable datasheet for OASE00377
Dilution 1:3000 dilution was sufficient for detection of PKBbeta in 20ug of HeLa cell lysate by ECL immunoblot analysis.
Target Reference 1. Downward J (1998). Curr Opin Cell Biol. 10(2): 262-7. 2. Brazil DP, and Hemmings BA (2001). Biochem Sci.
26(11):657-64.
3. Nicholson KM, Anderson NG (2002). Cell Signal. 14(5):
381-95.
4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts
Book, Protein-Serine Kinases Academic Press Limited,
San Diego, CA 418pps.
Storage Buffer TBS, 50% glycerol, 0.09% sodium azide
Gene Symbol AKT2
Official Gene Full Name v-akt murine thymoma viral oncogene homolog 2
NCBI Gene Id 208
Swissprot Id P31751
Protein Accession # NP_001617.1
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express AKT2.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express AKT2.
  1. What is the species homology for "Akt2 (PKB beta) Antibody (OASE00377)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus.".

  2. How long will it take to receive "Akt2 (PKB beta) Antibody (OASE00377)"?

    This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".

  3. What buffer format is "Akt2 (PKB beta) Antibody (OASE00377)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Akt2 (PKB beta) Antibody (OASE00377)"?

    This target may also be called "" in publications.

  5. What is the shipping cost for "Akt2 (PKB beta) Antibody (OASE00377)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Akt2 (PKB beta) Antibody (OASE00377)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Akt2 (PKB beta) Antibody (OASE00377)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Akt2 (PKB beta) Antibody (OASE00377)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "AKT2"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "AKT2"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "AKT2"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "AKT2"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "AKT2"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "AKT2"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

Write Your Own Review
You're reviewing:Akt2 (PKB beta) Antibody (OASE00377)
Your Rating
We found other products you might like!