Search Antibody, Protein, and ELISA Kit Solutions

Akt2 (PKB beta) Antibody (OASE00377)

  • Catalog#: OASE00377
  • Inquire
400ul
$310.00
Discontinued
Request Bulk Order Quote

Conjugation Options

OASE00377-FITC Conjugated

OASE00377-HRP Conjugated

OASE00377-Biotin Conjugated

Gene Symbol:
AKT2
NCBI Gene Id:
208
Official Gene Full Name:
v-akt murine thymoma viral oncogene homolog 2
Swissprot Id:
P31751
Protein Accession #:
NP_001617.1
Replacement Item:
This antibody may replace item sc-116831 from Santa Cruz Biotechnology.
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Rabbit immunoglobulin
Application:
WB, ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express AKT2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express AKT2.
Immunogen:
A five residue synthetic peptide based on the human Akt2, coupled to KLH
Reconstitution and Storage:
Store at -20C. Shipping in Blue Ice or 4C.
Concentration:
0.25mg/mL
Datasheets/Manuals:
Printable datasheet for OASE00377
Dilution:
1:3000 dilution was sufficient for detection of PKBbeta in 20ug of HeLa cell lysate by ECL immunoblot analysis.
Additional Information:
Background Info: Detects a 65kDa protein, corresponding to the molecular mass of Akt2 PKBbeta on SDS PAGE immunoblots. Does not react with PKBalpha (Akt1) or PKBγ (Akt3).
:::
Scientific Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the
phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
:::
Certificate of Analysis: A 13000 dilution was sufficient for detection of PKBbeta in 20ug of HeLa cell lysate by ECL immunoblot analysis.
Target Reference:
1. Downward J (1998). Curr Opin Cell Biol. 10(2): 262-7. 2. Brazil DP, and Hemmings BA (2001). Biochem Sci.
26(11):657-64.
3. Nicholson KM, Anderson NG (2002). Cell Signal. 14(5):
381-95.
4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts
Book, Protein-Serine Kinases Academic Press Limited,
San Diego, CA 418pps.
Storage Buffer:
TBS, 50% glycerol, 0.09% sodium azide

Product Reviews

Protocol:
Tips Information:

Tell us what you think about this item!

Write A Review
    Please, wait...