AKT1S1 ELISA Kit (Human) (OKEH07996)

Catalog No: OKEH07996

Size:96T

Human AKT1S1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	101-111% (n = 20)	104-114% (n = 20)	96-106% (n = 20)	81-81% (n = 20)
  EDTA plasma(n=5)	89-99% (n = 20)	96-108% (n = 20)	105-113% (n = 20)	105-115% (n = 20)
  heparin plasma(n=5)	91-102% (n = 20)	105-115% (n = 20)	106-115% (n = 20)	106-119% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology AKT1S1 ELISA Kit (Human) (OKEH07996) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AKT1S1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKT1S1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKT1S1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.3% (n = 20)
  Mean Inter-assay CV%: <=8.6% (n = 20)
• ELISA Kit Component:
  • AKT1S1 Microplate: 96 Wells (12 x 8 Well strips)
  • AKT1S1 Lyophilized Standard: 2
  • 100X Biotinylated AKT1S1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
  Subcellular Location: Cytoplasm Cytosol Found in the cytosolic fraction of the brain.
  Unigene: Hs.515542
  SMR: Q96B36
  String: 9606.ENSP00000341698
  KEGG: hsa:84335
  MIM: 610221
  Pfam: PF15798
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.057ng/mL
• Specificity: Natural and recombinant Human Proline-rich AKT1 substrate 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AKT1S1
• Gene Full Name: AKT1 substrate 1
• Alias Symbols: 40 kDa proline-rich AKT substrate;AKT1 substrate 1 (proline rich);Lobe;PRAS40;proline-rich AKT1 substrate 1.
• NCBI Gene Id: 84335
• Protein Name: Q96B36
• Description of Target: Proline-rich AKT1 substrate 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q96B36
• Protein Accession #: NP_001092102.1
• Nucleotide Accession #: NM_001098632.2