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- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for AKT1S1 Antibody (OACA07912) (OACA07912) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | Enzyme-linked immunosorbent assay|Immunohistochemistry |
| Reconstitution and Storage | -20°C or -80°C |
| Immunogen | Recombinant Human Proline-rich AKT1 substrate 1 protein (132-256AA) |
| Purification | Affinity Purified |
| Preservative | 0.02% sodium azide |
| Formulation | PBS with, 50% glycerol, pH7.3. |
| Gene Symbol | AKT1S1 |
|---|---|
| Gene Full Name | AKT1 substrate 1 |
| Alias Symbols | 40 kDa proline-rich AKT substrate;AKT1 substrate 1 (proline rich);Lobe;PRAS40;proline-rich AKT1 substrate 1. |
| NCBI Gene Id | 84335 |
| Protein Name | Proline-rich AKT1 substrate 1 |
| Description of Target | Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. |
| Uniprot ID | Q96B36 |
| Protein Accession # | NP_001092102 |
| Nucleotide Accession # | NM_001098632 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "AKT1S1 Antibody (OACA07912)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "AKT1S1 Antibody (OACA07912)"?
This item is available "Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks".
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What buffer format is "AKT1S1 Antibody (OACA07912)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "AKT1S1 Antibody (OACA07912)"?
This target may also be called "40 kDa proline-rich AKT substrate;AKT1 substrate 1 (proline rich);Lobe;PRAS40;proline-rich AKT1 substrate 1." in publications.
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What is the shipping cost for "AKT1S1 Antibody (OACA07912)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "AKT1S1 Antibody (OACA07912)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "AKT1S1 Antibody (OACA07912)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "AKT1S1 Antibody (OACA07912)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "AKT1S1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "AKT1S1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "AKT1S1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "AKT1S1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "AKT1S1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "AKT1S1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
