AKT1 ELISA Kit (Bovine) (OKEH07381)

Catalog No: OKEH07381

Size:96T

Bovine AKT1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology AKT1 ELISA Kit (Bovine) (OKEH07381) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AKT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKT1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.0% (n = 20)
  Mean Inter-assay CV%: <=8.8% (n = 20)
• ELISA Kit Component:
  • AKT1 Microplate: 96 Wells (12 x 8 Well strips)
  • AKT1 Lyophilized Standard: 2
  • 100X Biotinylated AKT1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation.
  Subcellular Location: Cytoplasm Nucleus Cell membrane Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A (By similarity). Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (By similarity).
  Unigene: Bt.4843
  SMR: Q01314
  KEGG: bta:280991
  Pfam: PF00169
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.099ng/mL
• Specificity: Natural and recombinant Bovine RAC-alpha serine/threonine-protein kinase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AKT1
• Gene Full Name: AKT serine/threonine kinase 1
• Alias Symbols: AKT;PKB alpha;Protein kinase B;protein kinase B alpha;RAC-alpha serine/threonine-protein kinase;RAC-PK-alpha.
• NCBI Gene Id: 280991
• Protein Name: Q01314
• Description of Target: RAC-alpha serine/threonine-protein kinase
• Uniprot ID: https://www.uniprot.org/uniprot/Q01314
• Protein Accession #: NP_776411.1
• Nucleotide Accession #: NM_173986.2