Human AKT1 Antibody : PE (Phospho-Ser473) (OAAQ00154)

Catalog No: OAAQ00154

Flow cytometric analysis of Jurkat cells treated with LY294002 (red) or with 20% FBS (green) using Phospho-Akt (Ser473) PE conjugated antibody AKTS473-B9. Cat. #OAAQ00154.

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 1X PBS, 0.09% NaN3, 0.2% BSA.
• Clonality: Monoclonal
• Clone: AktS473-B9
• Isotype: Rabbit IgG1k
• Host: Rabbit
• Conjugation: PE: Phycoerythrin
• Application: FC
• Reconstitution and Storage: 2-8C
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser473 of human phospho Akt1
• Purification: Protein A+G
• Target Post-Translational Modification: Phospho-Ser473
• Application Info: For flow cytometric staining, the suggested use of this reagent is 5 uL per million cells or 5 uL per 100 uL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Target Info

• Gene Symbol: AKT1
• Gene Full Name: AKT serine/threonine kinase 1
• Alias Symbols: AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA
• NCBI Gene Id: 207
• Protein Name: RAC-alpha serine/threonine-protein kinase
• Description of Target: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
• Uniprot ID: P31749
• Protein Accession #: NP_001014431.1
• Nucleotide Accession #: NM_001014431.1