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AKT1 Antibody (OALA02922)

50 ul
$539.00
In Stock
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Gene Symbol:
AKT1
Official Gene Full Name:
RAC-alpha serine/threonine-protein kinase
NCBI Gene Id:
207
Protein Name:
RAC-alpha serine/threonine-protein kinase
Swissprot Id:
P31749
Replacement Item:
This antibody may replace item sc-135829, HPA002891
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Delipidated and defibrinated
Application:
IHC-P, IF, WB, IP, ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express AKT1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express AKT1.
Immunogen:
Synthetic peptide from human AKT (AKT1/AKT2/AKT3).
Predicted Species Reactivity:
Human, Mouse, Rat, Chicken
Product Format:
Liquid
Reconstitution and Storage:
Store vial at -20C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Concentration:
75 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-AKT1 (OALA02922) antibody
Epitope:
C-Terminus
Warning:
For research use only.
Application Info:
IHC - Paraffin (1:100), Immunofluorescence (1:100 - 1:1000), Western blot (1:500 - 1:2000), Immunoprecipitation, ELISA (1:2000 - 1:10000)
Application Data:
Immunohistochemistry: OALA02922 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA02922 was determined to be 1:100.
Storage Buffer:
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.

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