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- $55: Antibody & Protein in US
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| Datasheets/Manuals | Printable datasheet for anti-AKT1 (OAAD00195) antibody |
|---|
| Predicted Species Reactivity | Human, Mouse, Rat, Chicken |
|---|---|
| Clonality | Monoclonal |
| Clone | IML-26 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | WB |
| Reconstitution and Storage | At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time. |
| Immunogen | Synthetic peptide corresponding to amino acids 461-477 of human PKBalpha/Akt1. |
| Purification | Purified by the goat anti-mouse IgG Affinity chromatography. |
| Specificity | Human, mouse, rat, chicken. No cross reactivity with other proteins. |
| Formulation | Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative. |
| Application Info | Western blot At 0.25-0.5ug/ml with the appropriate system to detect PKBalpha in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user. |
| Storage Buffer | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml. |
| Reference | 1 Staal, S. P.; Huebner, K.; Croce, C. M.; Parsa, N. Z.; Testa, J. R. : The AKT1 proto-oncogene maps to human chromosome 14, band q32. Genomics 2: 96-98, 1988. 2. Ackah, E.; Yu, J.; Zoellner, S.; Iwakiri, Y.; Skurk, C.; Shibata, R.; Ouchi, N.; Easton, R. M.; Galasso, G.; Birnbaum, M. J.; Walsh, K.; Sessa, W. C. : Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. J. Clin. Invest. 115: 2119-2127, 2005. 3. Chen, J.; Somanath, P. R.; Razorenova, O.; Chen, W. S.; Hay, N.; Bornstein, P.; Byzova, T. V. : Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo. Nature Med. 11: 1188-1196, 2005. |
| Gene Symbol | AKT1 |
|---|---|
| Gene Full Name | RAC-alpha serine/threonine-protein kinase |
| Alias Symbols | AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA |
| NCBI Gene Id | 207 |
| Protein Name | RAC-alpha serine/threonine-protein kinase |
| Description of Target | PKBalphaalso knows as V-AKT murine thymoma vial oncogene homolog 1(ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo. |
| Uniprot ID | P31749 |
| Protein Accession # | NP_001014431.1; NP_001014432.1; NP_005154.2; XP_005267458.1 |
| Nucleotide Accession # | NM_001014431.1; NM_001014432.1; NM_005163.2; XM_005267401.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "AKT1 Antibody (OAAD00195)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Chicken".
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How long will it take to receive "AKT1 Antibody (OAAD00195)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "AKT1 Antibody (OAAD00195)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "AKT1 Antibody (OAAD00195)"?
This target may also be called "AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA" in publications.
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What is the shipping cost for "AKT1 Antibody (OAAD00195)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "AKT1 Antibody (OAAD00195)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "AKT1 Antibody (OAAD00195)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "AKT1 Antibody (OAAD00195)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "AKT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "AKT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "AKT1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "AKT1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "AKT1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "AKT1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
